Journal: Genome Biology and Evolution

Article Title: Landscape of Fluid Sets of Hairpin-Derived 21-/24-nt-Long Small RNAs at Seed Set Uncovers Special Epigenetic Features in Picea glauca

doi: 10.1093/gbe/evw283

Figure Lengend Snippet: Comparisons of DCL3 homologs (A and B), pgRTL2 relative expression (C) and RPM scaled by the number of unique reads (D). Note : gene trees for DCL3 homologs in gymnosperms and several model angiosperms (A); components of DCL3 domains in Arabidopsis (general), Phoenix canariensis and Picea glauca (B); PAZ and dsRB represent Piwi–Argonaute–Zwille and double stranded RNA-binding domains, respectively; thick dashed line in (B) means the upstream of incomplete pgRTL2 mRNA may contain PAZ domain after mapped to its genome (PG29-v.4); RPM represents reads per million and if the absolute expression of the 24-nt sRNA exceeds 2,000 copies in a single library, it is not used for the calculation of RPM and RPM per unique read number. We excluded around 4-5 sRNA reads per library (see supplementary fig. S4 C , Supplementary Material online).

Article Snippet: To enrich sRNAs, total RNA samples underwent polyA selection using Miltenyi MultiMACS mRNA isolation kit (cat. 130-092-519) following the manufacturer’s protocol and the flowthrough (i.e., containing sRNA species without mRNA) was used for plate-based sRNA construction.

Techniques: Expressing, RNA Binding Assay